Hiv/Aids

Correction for Shanmugapriya et al., Dynactin 1 negatively regulates HIV-1 infection by sequestering the host cofactor CLIP170

MICROBIOLOGY Correction for “Dynactin 1 negatively regulates HIV-1 infection by sequestering the host cofactor CLIP170,” by Shanmugapriya Shanmugapriya, Eveline Santos da Silva, Jackson A. Campbell, Marie-Philipe Boisjoli, and Mojgan H. Naghavi, which published October 22, 2021; 10.1073/pnas.2102884118 (Proc. Natl. Acad. Sci. U.S.A. 118, e2102884118).

The authors note that, due to a printer’s error, Fig. 1 appeared incorrectly. In Fig. 1G, a blot for anti-flag was inadvertently removed during the production process. The corrected figure and its legend appear below. The online version was corrected on November 2, 2021.

Fig. 1.
Fig. 1.

DCTN1 binds to HIV-1 cores and regulates CLIP170 availability. (A) Representative WB analysis demonstrating that DCTN1 pelleted with in vitro–assembled HIV-1 CA-NC complexes. Levels of bound DCTN1 presented in Upper were quantified using Fiji and presented as a ratio of Bound/Input DCTN1 across three experimental replicates. (B and C) Representative WB analysis (B) showing that DCTN1-Flag, but not Flag control, also binds to fractions containing intact HIV-1 cores but not with Mock fractions. Quantification (C) of the levels of bound DCTN1 as a ratio of total protein across three experimental replicates. (DH) DCTN1 binds to HIV-1 CA-NC in a similar pattern to CLIP170 and reduces CLIP170 binding. Representative SIM images (D) of the Flag-tagged cyclophilin A (CypA-Flag), DCTN1-Flag, or Flag-CLIP170 bound to in vitro–assembled HIV-1 CA-NC complexes and stained with anti-p24 AG3.0 (Red) or anti-Flag (Green). (Scale bar, 2 μm.) (E) Quantification (n = 3) of the association of CypA-Flag or DCTN1-Flag with 109–96 CA-NC complexes, using untransfected lysates (Control) and EB1-Flag as controls (n = 87 and 123, respectively) for nonspecific background staining. (F) Quantification (n = 3) of Flag-CLIP170 association with 63–101 CA-NC complexes in the presence or absence of DCTN1 is shown. (G and H) CA-NC–binding assay using lysates from 293T expressing either Flag-CLIP170 treated with control or DCTN1 siRNAs (G) or Flag-CLIP170 or DCTN1 alone or constant amount of Flag-CLIP170 along with increasing amounts of DCTN1 (H). Quantification of the levels of bound CLIP170 as a ratio of total protein across experimental replicates (n = 3) are presented in G and H, Upper. Statistical analysis of all data were determined by one-way ANOVA, *P value ≤ 0.05, **P value ≤ 0.01, ***P value ≤ 0.001.


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